Nanofluidics

Ionic and water flow at the scales of nanometer and below. Next generation filtration membranes based on graphene.

Nanopore biophysics

Nanopore detection and analysis of DNA and proteins. Biopolymer detection

2D Materials

Electronic properties of 2D materials. Graphene composites for biomedical applications

Research

Research

Physical DNA Sequencing Using Graphene Nanopores

We aim to develop new physical methods for fast, single-molecule DNA sequencing based on graphene nanopores. Here, a long strand of individual DNA molecule is precisely controlled and manipulated using nanofluidic setup. The molecule is threaded through a nanometer scaled pore, with integrated electrical sensors detecting individual nucleotides in their natural sequential order.

Specific challenges are: (a) control of DNA position with sub-nm precision; (b) developing detector with sufficient nucleotide contrast; (c) achieving sufficient resolution along the DNA molecule to discern individual nucleobases

Advantages: Significant increase in sequencing speed and decrease in price will make full-genome DNA sequencing part of the general healthcare, through preventive and personalized medicine. Being single-molecule method, nanopore sequencing is uniquely suited for single-cell sequencing, removing the need for the DNA molecular amplifications.

References:
1. Proc. Nat. Acad. Sci. U.S.A. 110, 12192-12196 (2013).
2. Nature 467, 190–193 (2010).